Our key area of interest is in protein damage, which has far-reaching physiological implications in disease and health Advanced Glycation EndproductsGlycation is the non-enzymatic glycosylation of proteins, nucleotides and lipids by saccharide derivatives. It is now thought to contribute to the development of chronic vascular complications of diabetes, non-diabetic nephropathy, macrovascular disease, Alzheimer’s disease and ageing. Proteomics of protein glycation - the dicarbonyl proteomeSites of glycation in human albumin, haemoglobin, lens crystallins and collagen by dicarbonyl metabolites.Mapping of cellular proteins susceptibles to glycation by dicarbonyl metabolites and associated functional impairment.Molecular modelling of glycated proteins and prediction of hot spot sites susceptible to glycation damage. Protein glycation, oxidation and nitrosation biomarkers for disease diagnosis and progressionAssociation of protein biomarkers AGE with chronic complications of diabetes mellitus (retinopathy, neuropathy, nephropathy, cataract, microvascular disease), atherosclerosis, chronic renal insufficiency, Alzheimer’s disease, arthritis and ageing. Metabolic dysfunction and the development of diabetic complicationsBiochemical dysfunction associated with the development of diabetic complications (PKCβ activation, polyol pathway, activation of PARP, glycation, oxidative stress).Assessment of carbonyl stress in diabetes – measurement of plasma dicarbonyls and dicarbonyl glycation adducts in haemoglobin, plasma and urine.Evaluation of high-dose thiamine therapy for the prevention of diabetic complications.Evaluation of inducers of antioxidant response element -linked gene expression for the prevention of diabetic complications. Cancer chemoprevention and chemotherapyEvaluation of inducers of oxidant response element-linked gene expression for cancer chemoprevention.Role of glyoxalase I overexpression in multidrug resistance in cancer chemotherapy. Protein damage and proteolysis in ageingRole of protein damage, decline of enzymatic countermeasures and cellular proteolysis in ageing.Anti-ageing effects of glyoxalase I and antioxidant response element-linked gene expression.