Consultancy on analytical methods and data analysis for industry and academia.

Our experience encompasses a broad range of technical expertise

Mass Spectrometry

Quantitation of markers of proteins damage by glycation, oxidation and nitration by LC-MS/MS.
Metabolic mapping by LC-MS/MS.
Peptide mapping of protein damage by glycation, oxidation and nitration.
Proteomics of protein damage by glycation, oxidation and nitration.
Molecular modelling, kinetic and system modelling and bioinformatics

Quantitation of markers of proteins damage by glycation, oxidation and nitration by LC-MS/MS.
Metabolic mapping by LC-MS/MS.
Use of protein informatics to predict functional sites in proteins susceptible to damage.
Cell culture

Culture of human and murine cell lines. Isolation and culture of human blood cells (erythrocytes, neutrophils, monocytes, macrophages and lymphocytes). Isolation and culture of rat hepatocytes.

Glycoscience

Preparation and analysis of protein glycation adducts.
Studies of the enzymatic defence against glycation.
Metabolic and cytological analysis (biochemical and clinical)

Assay of enzyme activities (particularly glyoxalases, caspases, transketolase and other thiamine dependent enzymes), metabolic concentrations, immunoassay and immunoblotting, cell viability, cell growth, cell cycle, DNA, RNA and protein synthesis, glycolysis, immunoflourescence.
Signal transduction and apoptosis

Studies of tyrosine phosphorylation, protein tyrosine phosphatases, MAP kinases, transcription factors, caspase activities and inhibitors, and involvement of mitochondrial proteins.

Molecular biology techniques

cDNA propagation, vector customization, cell transfection for overexpression and siRNA gene silencing, PCR, real time RT-PCR, chromatin immunoprecipitation (ChIP), GFP-construct imaging, gene microarray and SNPs.
Synthetic biochemistry and physiological chemistry

Analytical standards for mass spectrometric assay of protein damage.
Analytical standards for mass spectrometric assay of physiological dicarbonyls.
Analytical standards for mass spectrometric assay of transcription factor activators.
General

Scintillation counting
Liquid chromatography
Gel electrophoresis
Light electron microscopy
Mass spectrometry
Electron spin resonance
High resolution nuclear magnetic resonance
Absorption & flourescence spectrophotometry
Electrochemical techniques
Statistics, experimental design & power analysis